Assessment of cytology techniques in oral mucosa of Sjögren's syndrome patients
Albiero, Eduardo; Gobbi, Carla; Yorio, Marcelo; Busamia, Beatriz
Facultad de Odontología, UNAM, publicado en Revista Odontológica Mexicana, y cosechado de Revistas UNAM
dor_id: 43364
506.#.#.a: Público
590.#.#.d: si
510.0.#.a: Consejo Nacional de Ciencia y Tecnología (CONACyT), Sistema Regional de Información en Línea para Revistas Científicas de América Latina, el Caribe, España y Portugal (Latindex), Scientific Electronic Library Online (SciELO)
561.#.#.u: http://www.odonto.unam.mx/
561.#.#.a: Facultad de Odontología, UNAM
650.#.4.x: Medicina y Ciencias de la Salud
336.#.#.b: article
336.#.#.3: Artículo Técnico-Profesional
336.#.#.a: Artículo
351.#.#.6: http://revistas.unam.mx/index.php/rom
351.#.#.b: Revista Odontológica Mexicana
351.#.#.a: Artículos
harvesting_group: RevistasUNAM
270.1.#.p: Revistas UNAM. Dirección General de Publicaciones y Fomento Editorial, UNAM en revistas@unam.mx
590.#.#.c: Open Journal Systems (OJS)
270.#.#.d: MX
270.1.#.d: México
590.#.#.b: Concentrador
883.#.#.u: http://www.revistas.unam.mx/front/
883.#.#.a: Revistas UNAM
590.#.#.a: Coordinación de Difusión Cultural, UNAM
883.#.#.1: https://www.publicaciones.unam.mx/
883.#.#.q: Dirección General de Publicaciones y Fomento Editorial, UNAM
850.#.#.a: Universidad Nacional Autónoma de México
856.4.0.u: http://revistas.unam.mx/index.php/rom/article/view/63578/55738
100.1.#.a: Albiero, Eduardo; Gobbi, Carla; Yorio, Marcelo; Busamia, Beatriz
524.#.#.a: Albiero, Eduardo, et al. (2018). Assessment of cytology techniques in oral mucosa of Sjögren's syndrome patients. Revista Odontológica Mexicana; Vol 22, No 1 Ing, 2018. Recuperado de https://repositorio.unam.mx/contenidos/43364
245.1.0.a: Assessment of cytology techniques in oral mucosa of Sjögren's syndrome patients
502.#.#.c: Universidad Nacional Autónoma de México
561.1.#.a: Facultad de Odontología, UNAM
264.#.0.c: 2018
264.#.1.c: 2018-02-28
506.1.#.a: La titularidad de los derechos patrimoniales de esta obra pertenece a las instituciones editoras. Su uso se rige por una licencia Creative Commons BY-NC-ND 4.0 Internacional, https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode.es, fecha de asignación de la licencia 2018-02-28, para un uso diferente consultar al responsable jurídico del repositorio por medio de danyelacarmona@gmail.com
884.#.#.k: http://revistas.unam.mx/index.php/rom/article/view/63578
041.#.7.h: eng
520.3.#.a: The aim of the present research project was to compare two techniques for oral cytology study (exfoliative cytology and impression cytology) in order to assess changes in oral mucosa which might allow non -invasive diagnosis of Sjögren’s syndrome cases (SS). Patients: 50 patients were selected, patients were paired by age and gender, and had been diagnosed with Sjögren’s syndrome according to criteria of the American-European Consensus. Patients were distributed into the following three experimental groups: Healthy control group (C), n = 14, Dry mouth and eyes group without SS, (ME) n = 13, and SS group n = 23. Material and methods: A cell harvesting brush (Cytobrush) was used for the exfoliative cytology procedure, sliding it along a glass plate and later fixating harvested cells in 95% ethanol. Cellulose acetate paper (Millipore Hawp 304®) was used for the impression cytology procedure. The paper was in 1 cm long stripes which were placed on the oral mucosa surface above upper vestibular groove; stripes were immobilized and pressure was applied for three seconds. Papanicolau (PAP) technique was used for dyeing. Morphology and histomorphology were assessed studying the following: cytoplasmic area (CA), nuclear areas (NA) nucleus-cytoplasm relationship (N:C) and amount of cells per square millimeter (mm2). Results: Both techniques revealed the following in C: isolated single-layered epithelial cells, basophils, normal central nuclei, 20 to 30 per mm2 , N:C ratio 1:8. In the eye and mouth group (EM): grouped and folded isolated cells, cytoplasm with eosinophilic predominance increase of cell amount to 40 per mm2 , nucleus-cytoplasm relationship N/C 1:4. SS patients showed the following: nuclear area with denser chromatin, 400 cells per mm2 , and 1:2 N/C relationship with respect to mouth and ears and control. Statistically significant differences wereobserved among groups in all studied characteristics. Conclusion: We can infer that impression cytology can be used in systemic and oral lesion’s diagnosis in patients afflicted with hyposalivation.
773.1.#.t: Revista Odontológica Mexicana; Vol 22, No 1 Ing (2018)
022.#.#.a: ISSN: 1870-199X
264.#.1.b: Facultad de Odontología, UNAM
758.#.#.1: http://revistas.unam.mx/index.php/rom/index
handle: 00a230627bd5e734
harvesting_date: 2019-02-06 00:00:00.0
856.#.0.q: application/pdf
last_modified: 2022-11-11 19:00:00
license_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode.es
license_type: by-nc-nd
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Albiero, Eduardo; Gobbi, Carla; Yorio, Marcelo; Busamia, Beatriz
Facultad de Odontología, UNAM, publicado en Revista Odontológica Mexicana, y cosechado de Revistas UNAM
Albiero, Eduardo, et al. (2018). Assessment of cytology techniques in oral mucosa of Sjögren's syndrome patients. Revista Odontológica Mexicana; Vol 22, No 1 Ing, 2018. Recuperado de https://repositorio.unam.mx/contenidos/43364