Evaluation of two RNA extraction methods in children’s saliva
Suárez Causado, Amileth; Madera Anaya, Meisser Vidal
Facultad de Odontología, UNAM, publicado en Revista Odontológica Mexicana, y cosechado de Revistas UNAM
dor_id: 43346
506.#.#.a: Público
590.#.#.d: si
510.0.#.a: Consejo Nacional de Ciencia y Tecnología (CONACyT), Sistema Regional de Información en Línea para Revistas Científicas de América Latina, el Caribe, España y Portugal (Latindex), Scientific Electronic Library Online (SciELO)
561.#.#.u: http://www.odonto.unam.mx/
561.#.#.a: Facultad de Odontología, UNAM
650.#.4.x: Medicina y Ciencias de la Salud
336.#.#.b: article
336.#.#.3: Artículo Técnico-Profesional
336.#.#.a: Artículo
351.#.#.6: http://revistas.unam.mx/index.php/rom
351.#.#.b: Revista Odontológica Mexicana
351.#.#.a: Artículos
harvesting_group: RevistasUNAM
270.1.#.p: Revistas UNAM. Dirección General de Publicaciones y Fomento Editorial, UNAM en revistas@unam.mx
590.#.#.c: Open Journal Systems (OJS)
270.#.#.d: MX
270.1.#.d: México
590.#.#.b: Concentrador
883.#.#.u: http://www.revistas.unam.mx/front/
883.#.#.a: Revistas UNAM
590.#.#.a: Coordinación de Difusión Cultural, UNAM
883.#.#.1: https://www.publicaciones.unam.mx/
883.#.#.q: Dirección General de Publicaciones y Fomento Editorial, UNAM
850.#.#.a: Universidad Nacional Autónoma de México
856.4.0.u: http://revistas.unam.mx/index.php/rom/article/view/63050/55428
100.1.#.a: Suárez Causado, Amileth; Madera Anaya, Meisser Vidal
524.#.#.a: Suárez Causado, Amileth, et al. (2017). Evaluation of two RNA extraction methods in children’s saliva. Revista Odontológica Mexicana; Vol 21, No 4 Ing. Recuperado de https://repositorio.unam.mx/contenidos/43346
245.1.0.a: Evaluation of two RNA extraction methods in children’s saliva
502.#.#.c: Universidad Nacional Autónoma de México
561.1.#.a: Facultad de Odontología, UNAM
264.#.0.c: 2017
264.#.1.c: 2018-01-10
506.1.#.a: La titularidad de los derechos patrimoniales de esta obra pertenece a las instituciones editoras. Su uso se rige por una licencia Creative Commons BY-NC-ND 4.0 Internacional, https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode.es, fecha de asignación de la licencia 2018-01-10, para un uso diferente consultar al responsable jurídico del repositorio por medio de danyelacarmona@gmail.com
884.#.#.k: http://revistas.unam.mx/index.php/rom/article/view/63050
041.#.7.h: eng
520.3.#.a: Objective: To standardize an RNA (ribonucleic acid) extraction protocol in children’s saliva specimens. Methods: The study was conducted on saliva specimens from 60 children who participated in the study with their parents’ authorization. Comparison of two RNA extraction methods was established; methods assessed concentration, quality and yield. Moreover, genic expression of GUSB gene was achieved with RT-PCR (reverse transcriptasepolymerase chain reaction). Data were analyzed through measurements of central tendency and dispersion, frequencies and percentages. T-Student and χ2 tests were used in order to differentiate between both methods (p < 0.05). Results: Analysis of cell amounts per saliva ml revealed a mean of 564,977.8 (SD = 246,678.6); a RNA in children’s saliva extraction method was standardized, specifically the method using RNeasy® Protect Saliva Mini Kit Qiagen exhibited better characteristics of RNA concentration (p = 0.0000) and yield (p = 0.0000) when compared to the method using QIAzol®; no RNA (p = 0.146) quality differences were found. Conclusions: RNA could be extracted from children’s saliva specimens, therefore, it is suggested to use saliva for the molecular analysis of different oral and systemic diseases.
773.1.#.t: Revista Odontológica Mexicana; Vol 21, No 4 Ing
022.#.#.a: ISSN: 1870-199X
264.#.1.b: Facultad de Odontología, UNAM
758.#.#.1: http://revistas.unam.mx/index.php/rom/index
handle: 6de112bbc9c83be2
harvesting_date: 2019-02-06 00:00:00.0
856.#.0.q: application/pdf
last_modified: 2022-11-11 19:00:00
license_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode.es
license_type: by-nc-nd
_deleted_conflicts: 2-fb59458ee2046899754ed14f0287a704
Suárez Causado, Amileth; Madera Anaya, Meisser Vidal
Facultad de Odontología, UNAM, publicado en Revista Odontológica Mexicana, y cosechado de Revistas UNAM
Suárez Causado, Amileth, et al. (2017). Evaluation of two RNA extraction methods in children’s saliva. Revista Odontológica Mexicana; Vol 21, No 4 Ing. Recuperado de https://repositorio.unam.mx/contenidos/43346