dor_id: 4149179

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650.#.4.x: Biología y Química

336.#.#.b: article

336.#.#.3: Artículo de Investigación

336.#.#.a: Artículo

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850.#.#.a: Universidad Nacional Autónoma de México

856.4.0.u: https://www.revistascca.unam.mx/rica/index.php/rica/article/view/RICA.2018.34.03.09/46773

100.1.#.a: Quintanar Gómez, Samuel; Abreu Corona, Arturo; Zamuido Pérez, Evelyn; Vargas Hernández, Genaro; Téllez Jurado, Alejandro; Gracida Rodríguez, Jorge Noel

524.#.#.a: Quintanar Gómez, Samuel, et al. (2018). PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM Cupriavidus necator WITH BEESWAX HYDROLYZATES AS CARBON SOURCE. Revista Internacional de Contaminación Ambiental; Vol. 34 Núm. 3, 2018; 467-474. Recuperado de https://repositorio.unam.mx/contenidos/4149179

245.1.0.a: PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM Cupriavidus necator WITH BEESWAX HYDROLYZATES AS CARBON SOURCE

502.#.#.c: Universidad Nacional Autónoma de México

561.1.#.a: Instituto de Ciencias de la Atmósfera y Cambio Climático, UNAM

264.#.0.c: 2018

264.#.1.c: 2018-07-31

653.#.#.a: biopolymers; DSC; mcl-PHA; NMR; fed-batch fermentation; biopolymers; DSC; mcl-PHA; NMR; fed-batch fermentation

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884.#.#.k: https://www.revistascca.unam.mx/rica/index.php/rica/article/view/RICA.2018.34.03.09

001.#.#.#: 105.oai:ojs.pkp.sfu.ca:article/52783

041.#.7.h: eng

520.3.#.a: This study demonstrates that using beeswax hydrolyzates (Hw), short- or medium-chain-length polyhydroxyalkanoates (scl-mcl-PHA) can be obtained by a three stages fed-batch fermentation system. The fed batch fermentations were conducted with a strain of Cupriavidus necator. Glucose and ammonium sulphate as carbon and nitrogen sources, respectively, were used in a first and second culture stages. In the third stage (production of scl-mcl-PHA) a fresh medium was fed with Hw as a carbon source at a concentration of 5 g/L, obtaining 8.91 g/L of intracellular scl-mcl-PHA and 13.64 g/L of biomass, which resulted in 65.3 % w/w of scl-mcl-PHA. Using nuclear magnetic resonance, the presence of scl and mcl monomers, 3-hydroxybutyrate (3HB) and 3-hydroxydecanoate (3HD) was determined. The percentage of comonomer incorporation different to 3HB was 4.6 mol %. A glass transition temperature (Tg) of –50 ºC and a melting temperature (Tm) of 79.8 ºC were determined for the scl-mcl-PHA (3HB-co-3HDD).

773.1.#.t: Revista Internacional de Contaminación Ambiental; Vol. 34 Núm. 3 (2018); 467-474

773.1.#.o: https://www.revistascca.unam.mx/rica/index.php/rica/index

022.#.#.a: ISSN impreso: 0188-4999

310.#.#.a: Trimestral

300.#.#.a: Páginas: 467-474

264.#.1.b: Instituto de Ciencias de la Atmósfera y Cambio Climático, UNAM

doi: https://doi.org/10.20937/RICA.2018.34.03.09

harvesting_date: 2023-11-08 13:10:00.0

856.#.0.q: application/pdf

file_creation_date: 2018-09-25 18:29:13.0

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245.1.0.b: PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM Cupriavidus necator WITH BEESWAX HYDROLYZATES AS CARBON SOURCE

last_modified: 2024-03-19 14:00:00

license_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode.es

license_type: by-nc

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Artículo

PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM Cupriavidus necator WITH BEESWAX HYDROLYZATES AS CARBON SOURCE

Quintanar Gómez, Samuel; Abreu Corona, Arturo; Zamuido Pérez, Evelyn; Vargas Hernández, Genaro; Téllez Jurado, Alejandro; Gracida Rodríguez, Jorge Noel

Instituto de Ciencias de la Atmósfera y Cambio Climático, UNAM, publicado en Revista Internacional de Contaminación Ambiental, y cosechado de Revistas UNAM

Licencia de uso

Procedencia del contenido

Cita

Quintanar Gómez, Samuel, et al. (2018). PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM Cupriavidus necator WITH BEESWAX HYDROLYZATES AS CARBON SOURCE. Revista Internacional de Contaminación Ambiental; Vol. 34 Núm. 3, 2018; 467-474. Recuperado de https://repositorio.unam.mx/contenidos/4149179

Descripción del recurso

Autor(es)
Quintanar Gómez, Samuel; Abreu Corona, Arturo; Zamuido Pérez, Evelyn; Vargas Hernández, Genaro; Téllez Jurado, Alejandro; Gracida Rodríguez, Jorge Noel
Tipo
Artículo de Investigación
Área del conocimiento
Biología y Química
Título
PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM Cupriavidus necator WITH BEESWAX HYDROLYZATES AS CARBON SOURCE
Fecha
2018-07-31
Resumen
This study demonstrates that using beeswax hydrolyzates (Hw), short- or medium-chain-length polyhydroxyalkanoates (scl-mcl-PHA) can be obtained by a three stages fed-batch fermentation system. The fed batch fermentations were conducted with a strain of Cupriavidus necator. Glucose and ammonium sulphate as carbon and nitrogen sources, respectively, were used in a first and second culture stages. In the third stage (production of scl-mcl-PHA) a fresh medium was fed with Hw as a carbon source at a concentration of 5 g/L, obtaining 8.91 g/L of intracellular scl-mcl-PHA and 13.64 g/L of biomass, which resulted in 65.3 % w/w of scl-mcl-PHA. Using nuclear magnetic resonance, the presence of scl and mcl monomers, 3-hydroxybutyrate (3HB) and 3-hydroxydecanoate (3HD) was determined. The percentage of comonomer incorporation different to 3HB was 4.6 mol %. A glass transition temperature (Tg) of –50 ºC and a melting temperature (Tm) of 79.8 ºC were determined for the scl-mcl-PHA (3HB-co-3HDD).
Tema
biopolymers; DSC; mcl-PHA; NMR; fed-batch fermentation; biopolymers; DSC; mcl-PHA; NMR; fed-batch fermentation
Idioma
eng
ISSN
ISSN impreso: 0188-4999

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