dor_id: 43301
506.#.#.a: Público
590.#.#.d: si
510.0.#.a: Consejo Nacional de Ciencia y Tecnología (CONACyT), Sistema Regional de Información en Línea para Revistas Científicas de América Latina, el Caribe, España y Portugal (Latindex), Scientific Electronic Library Online (SciELO)
561.#.#.u: http://www.odonto.unam.mx/
561.#.#.a: Facultad de Odontología, UNAM
650.#.4.x: Medicina y Ciencias de la Salud
336.#.#.b: article
336.#.#.3: Artículo Técnico-Profesional
336.#.#.a: Artículo
351.#.#.6: http://revistas.unam.mx/index.php/rom
351.#.#.b: Revista Odontológica Mexicana
351.#.#.a: Artículos
harvesting_group: RevistasUNAM
270.1.#.p: Revistas UNAM. Dirección General de Publicaciones y Fomento Editorial, UNAM en revistas@unam.mx
590.#.#.c: Open Journal Systems (OJS)
270.#.#.d: MX
270.1.#.d: México
590.#.#.b: Concentrador
883.#.#.u: http://www.revistas.unam.mx/front/
883.#.#.a: Revistas UNAM
590.#.#.a: Coordinación de Difusión Cultural, UNAM
883.#.#.1: https://www.publicaciones.unam.mx/
883.#.#.q: Dirección General de Publicaciones y Fomento Editorial, UNAM
850.#.#.a: Universidad Nacional Autónoma de México
856.4.0.u: http://revistas.unam.mx/index.php/rom/article/view/57725/51166
100.1.#.a: Cuin Macedo, Sergio Iván; Arroyo Cervantes, Roberto; Rodríguez Zaragoza, Diana Ened; Ruiz Reyes, Héctor; Calderón Rojas, Benigno Miguel
524.#.#.a: Cuin Macedo, Sergio Iván, et al. (2016). Proposal for experimental in vitro model to assess morphological alterations in erythrocytes exposed to 5.25% NaOCl. Revista Odontológica Mexicana; Vol 20, No 4-ING. Recuperado de https://repositorio.unam.mx/contenidos/43301
245.1.0.a: Proposal for experimental in vitro model to assess morphological alterations in erythrocytes exposed to 5.25% NaOCl
502.#.#.c: Universidad Nacional Autónoma de México
561.1.#.a: Facultad de Odontología, UNAM
264.#.0.c: 2016
264.#.1.c: 2017-01-26
506.1.#.a: La titularidad de los derechos patrimoniales de esta obra pertenece a las instituciones editoras. Su uso se rige por una licencia Creative Commons BY-NC-ND 4.0 Internacional, https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode.es, fecha de asignación de la licencia 2017-01-26, para un uso diferente consultar al responsable jurídico del repositorio por medio de danyelacarmona@gmail.com
884.#.#.k: http://revistas.unam.mx/index.php/rom/article/view/57725
041.#.7.h: eng
520.3.#.a: Introduction: Sodium hypochlorite (NaOCl) is the chemical agent most frequently used as irrigation solution during endodontic therapy. When extruded to periapical tissue, it is highly toxic. In endodontics, hemolysis caused by NaCOl has been proven using different models, nevertheless, there is little or no evidence of morphological alterations in the cellular membrane of erythrocytes. Objective: To propose an experimental model which might allow to assess morphological alterations suffered by erythrocytes when they are exposed to NaOCl used in the dental practice by means of high resolution scanning electron microscopy (SEM). Materials and methods: In the present study, 20 mL of peripheral blood were obtained and deposited in tubes with EDTA (ethylenediaminetetraacetic acid) anticoagulant. Rinses were conducted with a phosphate buffer solution (Evan’s solution). Several dilutions of the erythrocyte sample were prepared (1:1, 1:2, 1:4, 1:8 and 1:16); 100 μL of each of these dilutions was obtained to be then confronted with 100 μL of dental use 5.25% NaOCl (Viarzoni-T, Medental®); 0.5 μL of these samples were taken to then be deposited in a sample holder made of Zn-Cu alloy which was subjected to a process of Cu ion metallization bath, following the old Spluttering method. Microphotographs were obtained with SEM. Results: Erythrocytes with alteration type anisocytosis and poikilocytosis (stomatocytes, elliptocytes and discocytes) were observed. Some structural characteristics of NaOCl crystals were equally observed. Conclusion: This experimental model allowed assessment of morphological changes experienced by erythrocytes when exposed to 5.25% NaOCl.
773.1.#.t: Revista Odontológica Mexicana; Vol 20, No 4-ING
022.#.#.a: ISSN: 1870-199X
264.#.1.b: Facultad de Odontología, UNAM
758.#.#.1: http://revistas.unam.mx/index.php/rom/index
handle: 00f691ccf6b2300e
harvesting_date: 2019-02-06 00:00:00.0
856.#.0.q: application/pdf
last_modified: 2022-11-11 19:00:00
license_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode.es
license_type: by-nc-nd
_deleted_conflicts: 2-12c0baf0777aff0361fdd33b96b141e3
No entro en nada
No entro en nada 2